RAD52 Rabbit Polyclonal Antibody

CAT#: AP20268PU-N

RAD52 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of Rad52 antibody (AP20268PU-N) in extracts from 293 cells.

• 

  Immunohistochemistry analyzes of Rad52 antibody (AP20268PU-N) in paraffin-embedded human testis tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of Rad52 protein.
• Formulation: Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen; purity is > 95% (by SDS-PAGE)
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: ~ 46 kDa
• Gene Name: Homo sapiens RAD52 homolog, DNA repair protein (RAD52), transcript variant 1
• Database Link:
  Entrez Gene 19365 MouseEntrez Gene 5893 Human
• Background: DNA double-strand breaks are generated by ionizing radiation and endogenously produced radicals, and they often are repaired through the RAD52 homologous recombination pathway. The RAD52 family includes RAD51, RAD52, RAD54, RAD54B and MRE11 genes. Rad51 and Rad52 proteins perform the key steps in homologous recombination (HR), including the search for DNA homology and strand exchange, through similar mechanisms. Mre11 functions in both non-homologous end joining, and meiotic HR, and it is essential in mitosis for chromosome maintenance. Rad54 belongs to the SWI2/SNF2 subfamily of ATPases, which includes the DNA helicases involved in replication, recombination, and repair, as it contains seven amino acid sequence motifs that are largely conserved. Rad54 ATPase activity is dependent on double-stranded (ds) DNA, and the ATPase activity of Rad54 is not absolutely required for its DNA repair function, suggesting that these activities occur at distinct regions of the molecule. RAD54B is significantly homologous to the RAD54 recombination gene. Expression of RAD54B is highest in testis and spleen, which are active in both meiotic and mitotic recombination.
• Synonyms: RAD52

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Homologous recombination