ATP citrate lyase (ACLY) Rabbit Polyclonal Antibody

CAT#: AP20289PU-N

ATP citrate lyase (ACLY) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of ATP-citrate synthase antibody in extracts from COS7 cells.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunoflourescence: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 430-480 of Human ATP-Citrate synthase.
• Specificity: This antibody detects endogenous levels of ATP-citrate synthase protein.
• Formulation: Phosphate buffered saline (PBS), pH~7.2 with 0.05% Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 120 kDa
• Gene Name: Homo sapiens ATP citrate lyase (ACLY), transcript variant 1
• Database Link:
  Entrez Gene 24159 RatEntrez Gene 104112 MouseEntrez Gene 47 Human
• Background: ATP citrate lyase (ACL) is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of four identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. One of these products, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. NDPK has been found to phosphorylate ACL and insulin to increase phosphorylation of ACL.
• Synonyms: ACLY

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Citrate cycle (TCA cycle), Metabolic pathways