Vitamin D Receptor (VDR) Rabbit Polyclonal Antibody

CAT#: AP20319PU-N

Vitamin D Receptor (VDR) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of VDR antibody (Cat.-No.: AP20319PU-N) in extracts from Hela cells.

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  Immunohistochemistry analysis of Vitamin D3 receptor / NR1I1 antibody (Cat.-No.: AP20319PU-N) in paraffin-embedded human thyroid gland tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of VDR protein.
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: One year from despatch.

• Predicted Protein Size: ~48.0 kDa
• Gene Name: Homo sapiens vitamin D (1,25- dihydroxyvitamin D3) receptor (VDR), transcript variant 1
• Database Link:
  Entrez Gene 7421 Human
  UniProt ID P11473
• Background: The active metabolite of vitamin D modulates the expression of a wide variety of genes in a developmentally-specific manner. This secosteroid hormone can up- or downregulate the expression of genes involved in a diverse array of responses such as proliferation, differentiation and calcium homeostasis. 1,25-(OH)2-vitamin D3 exerts its effects through interaction with the vitamin D receptor (VDR), a member of the superfamily of hormone-activated nuclear receptors. In its ligand-bound state, the VDR forms heterodimers with the 9- cis retinoic acid receptor, RXR, and affects gene expression by binding specific DNA sequences known as hormone response elements, or HREs. In addition to regulating the above-mentioned cellular responses, 1,25-(OH)2-vitamin D3 exhibits antiproliferative properties in osteosarcom,a melanom,a colon carcinoma and breast carcinoma cells.
• Synonyms: VDR, 1,25-dihydroxyvitamin D3 receptor

Reference Data

• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors