Ku70 (XRCC6) Rabbit Polyclonal Antibody

CAT#: AP20349PU-N

Ku70 (XRCC6) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of Ku70 antibody in extracts from HeLa cells.

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  Immunohistochemistry analyzes of Ku70 antibody in paraffin-embedded human liver carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to the N-terminus of Human Ku-70.
• Specificity: This antibody detects endogenous levels of Ku70 protein.
• Formulation: Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen; purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 70 kDa
• Gene Name: Homo sapiens X-ray repair cross complementing 6 (XRCC6), transcript variant 1
• Database Link:
  Entrez Gene 14375 MouseEntrez Gene 2547 Human
  UniProt ID P12956
• Background: The Ku protein is localized in the nucleus and is composed of subunits referred to as Ku-70 (or p70) and Ku-86 (or p86), which is also known by the synonym Ku-80 (or p80). Ku was first described as an autoantigen to which antibodies were produced in a patient with scleroderma-polymyositis overlap syndrome, and was later found in the sera of patients with other rheumatic diseases. Both subunits of the Ku protein have been cloned, and a number of functions have been proposed for Ku, including cell signaling, DNA replication and transcriptional activation. Ku is involved in Pol II-directed transcription by virtue of its DNA-binding activity, serving as the regulatory component of the DNA-associated protein kinase that phosphorylates Pol II and transcription factor Sp. Ku proteins also activate transcription from the U1 small nuclear RNA and the human transferrin receptor gene promoters. A Ku-related protein designated the enhancer 1 binding factor (E1BF), composed of two subunits, has been identified as a positive regulator of RNA polymerase I transcription initiation.
• Synonyms: G22P1, TLAA, CTCBF, CTC75

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Non-homologous end-joining