Casein Kinase 1 alpha (CSNK1A1) Rabbit Polyclonal Antibody

CAT#: AP20367PU-N

Casein Kinase 1 alpha (CSNK1A1) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of Casein Kinase I a antibody (Cat.-No.: AP20367PU-N) in extracts from HuvEc cells.

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  Immunohistochemistry analyzes of Casein Kinase I a antibody (Cat.-No.: AP20367PU-N) in paraffin-embedded human colon carcinoma tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of Casein Kinase I α protein.
• Formulation: Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen; purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 35 kDa
• Gene Name: Homo sapiens casein kinase 1 alpha 1 (CSNK1A1), transcript variant 2
• Database Link:
  Entrez Gene 93687 MouseEntrez Gene 113927 RatEntrez Gene 1452 Human
  UniProt ID P48729
• Background: Casein kinase I (also designated CKI) and casein kinase II (CKII) compose a family of serine/threonine protein kinases which are present in all eukaryotes examined to date. Casein kinase I family members, which include casein kinase Iα, Iγ, Iδ and Iε, have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. CKII is usually expressed as a tetrameric complex consisting of either an α2β2 or an αα‘β2 structure. The a catalytic subunit is stimulated by the β regulatory subunit, which undergoes autophosphorylation. Casein kinase II activity is high in the cytosol and nucleus of proliferating and differentiating cells. Casein kinase II is known to phosphorylate more than 100 different substrates including nuclear oncoproteins, transcription factors and enzymes involved in DNA metabolism.
• Synonyms: Casein kinase I alpha, CKI-alpha, CK1

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase
• Protein Pathways: Hedgehog signaling pathway, Wnt signaling pathway