LIM kinase 2 (LIMK2) Rabbit Polyclonal Antibody

CAT#: AP20423PU-N

LIM kinase 2 (LIMK2) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of AP20423PU-N LIMK2 antibody in extracts from HepG2 cells.

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  Immunohistochemistry: AP20423PU-N LIMK2 antibody staining of Paraffin-Embedded Human brain tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of LIMK2 protein.
  (region surrounding Lys491)
• Formulation: Phosphate buffered saline (PBS), pH~7.2 containing 0.05% Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
• Concentration: 1.0 mg/ml
• Purification: Affinity-Chromatography using epitope-specific immunogen.
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 72 kDa
• Gene Name: Homo sapiens LIM domain kinase 2 (LIMK2), transcript variant 2a
• Database Link:
  Entrez Gene 16886 MouseEntrez Gene 29524 RatEntrez Gene 3985 Human
• Background: Proteins containing LIM motifs are typically involved in cell fate determination and growth control. A family of proteins designated LIM kinases, incuding LIMK-1 and LIMK-2, has been identified. LIMK-1 has been shown to regulate the stabilization of F-Actin structures and Cofilin activity, indicating that LIMK-1 plays a role in a signaling pathway involved in the regulation of cell motility and morpho-genesis. LIMK-1 inhibits neuronal differentiation of PC12 cells, and it is thought to act by interfering with events downstream of MAPK activation. Expression patterns of LIMK-1 and LIMK-2 suggest that these proteins may have different functions during development. A truncated form of LIMK-2 has been identified in adult testis that is thought to arise from an alternative initiation exon.
• Synonyms: LIMK2, LIM domain kinase 2, LIMK-2, EC=2.7.11.1

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton