PER3 Rabbit Polyclonal Antibody

CAT#: AP20426PU-N

PER3 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of Per3 antibody (Cat.-No.: AP20426PU-N) in extracts from Jurkat cells treated with insulin 0.01U.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of Per3 protein.
  (region surrounding Pro2)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~43 kDa
• Gene Name: Homo sapiens period circadian clock 3 (PER3), transcript variant 4
• Database Link:
  Entrez Gene 8863 Human
  UniProt ID P56645
• Background: Biological timepieces called circadian clocks are responsible for the regulation of hormonal rhythms, sleep cycles and other behaviors. The superchiasmatic nucleus (SCN), which is located in the brain, was the first mammalian circadian clock to be discovered. A number of transcription factors appearing to be molecular components of the SCN clock have been identified. Mutations within the Clock gene increase the length of the endogenous period and cause a loss of rhythmicity of circadian oscillations. Three mammalian period proteins, designated Per1, Per2 and Per3, exhibit circadian rhythyms in the SCN.During subjective night, Per1 and Per2 RNA levels increase in response to light pulses while Per3 RNA levels show no change in response to light pulses. Tim, for timeless, interacts with Per1 as well as Per2; and Tim and Per1 negatively regulate Clock-BMAL1-induced transcription.
• Synonyms: Period circadian protein homolog 3, hPER3, Circadian clock protein PERIOD 3, GIG13

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Circadian rhythm - mammal