PFKL Rabbit Polyclonal Antibody

CAT#: AP20430PU-N

PFKL rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of PFK-1 antibody (Cat.-No.: AP20430PU-N) in extracts from A549 and raw264.7 cell.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of PFK-C protein.
  (region surroudning Gln365)
• Formulation: Phosphate buffered saline (PBS), pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen; purity is > 95% (by SDS-PAGE)
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 85 kDa
• Gene Name: Homo sapiens phosphofructokinase, liver type (PFKL), transcript variant 2
• Database Link:
  Entrez Gene 18641 MouseEntrez Gene 25741 RatEntrez Gene 5211 Human
• Background: The PFKP gene encodes the platelet isoform of phosphofructokinase (PFK). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The PFKP gene, which maps to chromosome 10p, is also expressed in fibroblasts. See also the muscle and liver isoforms of phosphofructokinase, which map to chromosomes 12q13 and 21q22, respectively. Vora determined that full tetrameric phophofructokinase enzyme expressed in platelets can be composed of subunits P4, P3L, and P2L2.
• Synonyms: Phosphofructokinase 1, 6-phosphofructokinase liver, PFK-B, Phosphohexokinase

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Fructose and mannose metabolism, Galactose metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Pentose phosphate pathway