CDC42 Binding Protein Kinase Beta (CDC42BPB) Rabbit Polyclonal Antibody

CAT#: AP20465PU-N

CDC42 Binding Protein Kinase Beta (CDC42BPB) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of MRCKβ antibody (Cat.-No.: AP20465PU-N) in extracts from COLO cell.

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  Immunohistochemistry (IHC) analyzes of MRCKbeta antibody (Cat.-No.: AP20465PU-N) in paraffin-embedded human brain tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of MRCKbeta protein.
  (region surrounding Phe1665)
• Formulation: Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 194 kDa
• Gene Name: Homo sapiens CDC42 binding protein kinase beta (CDC42BPB)
• Database Link:
  Entrez Gene 113960 RatEntrez Gene 217866 MouseEntrez Gene 9578 Human
  UniProt ID Q9Y5S2
• Background: Protein kinases comprise a large group of encoded factors that regulate cellular processes by catalyzing the transfer of a phosphate group to a hydroxyl acceptor in serine, threonine or tyrosine residues. Myotonic dystrophy kinaserelated Cdc42-binding (DMPK-like) kinases-alpha and beta (MRCK-alpha, beta) contain a cysteine-rich motif and a putative pleckstrin homology domain. MRCKs can phosphorylate nonmuscle Myosin light chain and influences Actin-Myosin contractility. MRCK-alpha can phosphorylate and activate LIM kinases downstream of Cdc42, which leads to inactivation of ADF/Cofilin and to Actin cytoskeletal reorganization. MRCK-alpha can also influence neurite outgrowth promoted by Cdc42 and Rac.
• Synonyms: MRCK beta, KIAA1124, DMPK-like beta

Reference Data

• Protein Families: Druggable Genome, Protein Kinase