14 3 3 eta (YWHAH) Rabbit Polyclonal Antibody

CAT#: AP20467PU-N

14 3 3 eta (YWHAH) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of 14-3-3 eta antibody at 1/500 dilution: Lane 1: MCF-7 cell lysate. Lane 2: sp2/0 cell lysate. Lane 3: H9C2 cell lysate.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 50-100 of Human 14-3-3 η.
• Specificity: This antibody detects endogenous levels of 14-3-3 theta protein.
  (region surrounding Lys81)
• Formulation: Phosphate Buffered Saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~28 kDa
• Gene Name: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein eta
• Database Link:
  Entrez Gene 22629 MouseEntrez Gene 25576 RatEntrez Gene 7533 Human
  UniProt ID Q04917
• Background: 14-3-3 eta (YWHAH) belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine or phosphothreonine-containing proteins. YWHAH is an adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. It binds to a large number of partners and binding generally results in the modulation of the activity of the binding partner. The YWHAH gene contains a 7 bp repeat sequence in its 5' UTR, and changes in the number of this repeat has been associated with early-onset schizophrenia.
• Synonyms: Protein AS1, YWHAH, YWHA1

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Cell cycle, Neurotrophin signaling pathway, Oocyte meiosis