Iba1 (AIF1) Rabbit Polyclonal Antibody

CAT#: AP20480PU-N

Iba1 (AIF1) rabbit polyclonal antibody, Aff - Purified

Product Images

Western Blot Analysis using of ant AIF1 on MCF7 cells (lane 1), Mouse liver tissue lysate (lane 2) and rat heart tissue lysate (land 3)

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
  Immunofluorescence: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide in the region aa 50-100 of human AIF1
• Specificity: This antibody detects endogenous levels of AIF-M1 protein.
  (region surrounding Tyr85)
• Formulation: Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 67 kDa
• Gene Name: Homo sapiens allograft inflammatory factor 1 (AIF1), transcript variant 3
• Database Link:
  Entrez Gene 11629 MouseEntrez Gene 29427 RatEntrez Gene 199 Human
  UniProt ID P55008
• Background: Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF). Like the critical molecules, cytochrome c and caspase 9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase 9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli. AIF is highly conserved between human and mouse and widely expressed.
• Synonyms: AIF-1, Protein G1, IBA1, Allograft inflammatory factor 1

Reference Data

• Protein Families: Druggable Genome