SIAH2 Rabbit Polyclonal Antibody

CAT#: AP20493PU-N

SIAH2 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of Siah-2 antibody (Cat.-No.: AP20493PU-N) in extracts from COLO cells.

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  Immunohistochemistry (IHC) analyzes of Siah-2 antibody (Cat.-No.: AP20493PU-N) in paraffin-embedded human heart tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunhistochemistry on paraffin sections: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of Siah-2 protein.
  (region surrounding Glu266)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 38 kDa
• Gene Name: Homo sapiens siah E3 ubiquitin protein ligase 2 (SIAH2)
• Database Link:
  Entrez Gene 20439 MouseEntrez Gene 140593 RatEntrez Gene 6478 Human
• Background: SIAH-2 (seven in absentia homolog 2) is an E3 ligase that catalyzes ubiquitination and proteasome-mediated degradation of protein substrates. SIAH-2 encodes a 324 amino acid protein that shares 77% identity with human SIAH-1 and 68% identity with the Drosophila sina (seven in absentia) gene, on which development of the Drosophila R7 photoreceptor is dependent. SIAH-2 targets TRAF2 (which regulates cell responses to stress and cytokines through the regulation of key stress-signaling cascades) for degradation under stress conditions such as hypoxia. It targets HIF-1alpha prolyl hydroxylase 3 (PHD3) for degradation upon exposure to hypoxic conditions, which coincides with an increase in SIAH-2 transcription. SIAH-2 can decrease TNF-alpha-dependent induction of JNK activity and transcriptional activation of NFkappaB. SIAH-2 null mice subjected to hypoxia display an impaired respiratory response and reduced levels of hemoglobin.
• Synonyms: Siah-2

Reference Data

• Protein Families: Druggable Genome, Transcription Factors