NFYC Rabbit Polyclonal Antibody

CAT#: AP20495PU-N

NFYC rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of NF-YC antibody (Cat.-No.: AP20495PU-N) in extracts from HeLa.

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  Immunohistochemistry (IHC) analyzes of NF-YC antibody (Cat.-No.: AP20495PU-N) in paraffin-embedded human liver carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of NF-YC protein.
  (region surrounding Trp85)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 50 kDa
• Gene Name: Homo sapiens nuclear transcription factor Y subunit gamma (NFYC), transcript variant 2
• Database Link:
  Entrez Gene 18046 MouseEntrez Gene 25337 RatEntrez Gene 4802 Human
• Background: The CCAAT-binding factor NF-Y is a heteromeric transcription factor that specifically binds to CCAAT sequences in many eukaryotic genes. NF-Y is made up of three subunits, NF-YA, NF-YB and NF-YC. All three components are necessary for DNA binding. In each NF-Y subunit, the segment needed for formation of the NF-Y-DNA complex is conserved from yeast to human. These conserved segments are homologous to the histone-fold motif of eukaryotic histones. The DNA binding domains of the NF-YB and NF-YC subunits have been suggested to interact through a protein-protein histone-fold “handshake” motif in a manner analogous to the histone proteins, H2B and H2A, respectively.
• Synonyms: CBF-C; CBFC; DKFZp667G242; FLJ45775; H1TF2A; HAP5; HSM; NF-YC; OTTHUMP00000009208; OTTHUMP00000009209; OTTHUMP00000009211; OTTHUMP00000009212

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: Antigen processing and presentation