c Kit (KIT) Rabbit Polyclonal Antibody

CAT#: AP20497PU-N

c Kit (KIT) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of CD117/c-Kit antibody at 1/500 dilution in: Lane 1 : MCF-7 cell lysate. Lane 2 : sp2/0 cell lysate. Lane 3 : NIH-3T3 cell lysate.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide surrounding amino acids 901-950 Glu930 of Human c-Kit.
• Specificity: This antibody detects endogenous levels of c-Kit protein. (region surrounding Glu930)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 120, 145 kDa
• Gene Name: Homo sapiens KIT proto-oncogene receptor tyrosine kinase (KIT), transcript variant 1
• Database Link:
  Entrez Gene 16590 MouseEntrez Gene 3815 Human
• Background: c-Kit is a transmembrane tyrosine kinase encoded by the cKit proto oncogene. c-Kit acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. Mutations in c-Kit have been found to be important for tumor growth and progression in a variety of cancers including mast cell diseases, gastrointestinal stromal tumor, acute myeloid leukemi,a Ewing sarcoma and lung cancer. Phosphorylation at tyrosine 721 of c-Kit allows binding and activation of PI3 kinase.
• Synonyms: SCFR, KIT

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Protein Kinase, Stem cell - Pluripotency, Transmembrane
• Protein Pathways: Acute myeloid leukemia, Cytokine-cytokine receptor interaction, Endocytosis, Hematopoietic cell lineage, Melanogenesis, Pathways in cancer