TCF3 / E2A (TCF3) Rabbit Polyclonal Antibody

CAT#: AP20520PU-N

TCF3 / E2A (TCF3) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of TCF-3 antibody (Cat.-No.: AP20520PU-N) in extracts from hela cells.

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  Immunohistochemistry (IHC) analyzes of TCF-3 antibody (Cat.-No.: AP20520PU-N) in paraffin-embedded human brain tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of TCF-3 protein.
  (region surrounding asn601)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 15 mM sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 60 kDa
• Gene Name: Homo sapiens transcription factor 3 (TCF3), transcript variant 2
• Database Link:
  Entrez Gene 21423 MouseEntrez Gene 171046 RatEntrez Gene 6929 Human
• Background: Heterodimers between TCF3 and tissue specific basic helix loop helix (bHLH) proteins play major roles in determining tissue specific cell fate during embryogenesis, like muscle or early B cell differentiation. Dimers bind DNA on E box motifs: 5' CANNTG 3'. Binds to the kappa E2 site in the kappa immunoglobulin gene enhancer.
• Synonyms: Transcription factor 3, TCF-3, Transcription factor E2-alpha, ITF1, ITF-1

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transcription Factors