ER81 (ETV1) Rabbit Polyclonal Antibody

CAT#: AP20524PU-N

ER81 (ETV1) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of ER81 Antibody (Cat.-No.: AP20524PU-N) in extracts from HUVEC cells treated with PMA (125ng).

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of ER81 protein.
  (region surrounding asn23)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction. Synthetic peptide ER81. (Note: the amino acid sequence is proprietary). The purity is > 95%.
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml in DI water
• Purification: Affinity Chromatography using epitope-specific immunogen
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Predicted Protein Size: ~ 60 kDa
• Gene Name: Homo sapiens ets variant 1 (ETV1), transcript variant 1
• Database Link:
  Entrez Gene 14009 MouseEntrez Gene 362733 RatEntrez Gene 2115 Human
• Background: Several members of the Ets gene family encode sequence-specific DNA binding proteins that recognize DNA sequences with a centrally located 5'-GGAA-3' element. All of the Ets proteins recognize the same central core sequence but each protein interacts with unique sequences that flank this core. ER81 (also designated ETV1) binds the motif 5'-CGGAA/T-3'. ER81 is highly expressed in brain, testis, lung and heart. ER81 is also moderately expressed in spleen, pancreas, colon and small intestine. During development, ER81 displays a unique expression patterns which suggests that this transcriptional factor might play an important role in organogenesis. ERK-1 activates ER81 transcriptional activity while MAPKAP kinase 2 inhibits ER81.
• Synonyms: ETS translocation variant 1, Ets-related protein 81

Reference Data

• Protein Families: ES Cell Differentiation/IPS, Transcription Factors