AMPK gamma 1 (PRKAG1) Rabbit Polyclonal Antibody

CAT#: AP20529PU-N

AMPK gamma 1 (PRKAG1) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of AMPKgamma1/2/3 antibody (Cat.-No.: AP20529PU-N) in extracts from 293 cells.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunofluorescence: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of AMPKgamma1/2/3 protein.
  (region surrounding Lys78)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 15 mM sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~40,42 kDa
• Gene Name: Homo sapiens protein kinase AMP-activated non-catalytic subunit gamma 1 (PRKAG1), transcript variant 1
• Database Link:
  Entrez Gene 19082 MouseEntrez Gene 25520 RatEntrez Gene 5571 Human
• Background: The 5-AMP activated protein kinase (AMPK), a member of the SNF1 (sucrose non fermentor) kinase family, is a heterotrimeric protein comprise of alpha (63 kDa), beta (30 kDa) and gamma (38 kDa) subunits. The alpha subunit is the catalytic subunit, while beta and gamma are non catalytic subunits (although they have been found to interact with the active subunit in liver). AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase. AMPK is activated by AMP and can be also regulated by treatment with purified protein phosphatase in vitro.
• Synonyms: AMPKg, PRKAG1, PRKAG2, PRKAG3, H91620p, AMPKG3, AMPK gamma-1, AMPK gamma-2, AMPK gamma-3

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Adipocytokine signaling pathway, Hypertrophic cardiomyopathy (HCM), Insulin signaling pathway