DOK6 Rabbit Polyclonal Antibody

CAT#: AP20534PU-N

DOK6 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of Dok-6 pAb in extracts from COLO205 cells.

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  Immunohistochemistry (IHC) analyzes of Dok-6 pAb in paraffin-embedded human brain tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
  Immunofluorescence: 1/50 - 1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of Dok-6 protein.
  (region surrounding Gly130)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Predicted Protein Size: ~ 43 kDa
• Gene Name: Homo sapiens docking protein 6 (DOK6)
• Database Link:
  Entrez Gene 623279 MouseEntrez Gene 220164 Human
• Background: The downstream of kinase family (Dok1-6) are members of a class of “docking” proteins that include the tyrosine kinase substrates IRS-1 and Cas, which contain multiple tyrosine residues and putative SH2 binding sites. Dok-4, Dok-5 and Dok-6 are more similar to each other than to the other Dok family members, and may constitute a subfamily of the DOK genes. Dok-5 is a tyrosine kinase substrate that enhances c-Ret-dependent activation of mitogen-activated protein kinase (MAPK). Dok-5 transcript is abundant in muscle and increases during T cell activation. Dok-5 protein undergoes tyrosine phosphorylation in response to insulin and insulin-like growth factor-1. The gene encoding human Dok-5 maps to chromosomal location 20q13.2. Dok-6 is highly expressed in the developing central nervous system. It associates with Ret to transduce Ret-mediated processes such as axonal projection.
• Synonyms: Docking protein 6

Reference Data

• Protein Families: Druggable Genome