c Abl (ABL1) Rabbit Polyclonal Antibody

CAT#: AP20540PU-N

c Abl (ABL1) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of c-Abl antibody Cat.-No. AP20540PU-N in extracts from 293 cells.

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  Western blot analysis of c-Abl antibody Cat.-No AP20540PU-N at 1/500 dilution in HepG2 whole cell lysate (Lane 1), Mouse (Lane 2) and Rat (Lane 3) liver tissue lysates.

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  Immunohistochemistry analysis of c-Abl antibody Cat.-No. AP20540PU-N in paraffin-embedded human brain tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of c-Abl protein.
  (region surrounding Glu883)
• Formulation: PBS, pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 123 kDa
• Gene Name: Homo sapiens ABL proto-oncogene 1, non-receptor tyrosine kinase (ABL1), transcript variant a
• Database Link:
  Entrez Gene 11350 MouseEntrez Gene 311860 RatEntrez Gene 25 Human
  UniProt ID P00519
• Background: The Abl oncogene was initially identified as the viral transforming gene of Abelson murine leukemia virus (A-MuLV). The major translational product of c-Abl has been identified as a protein with tyrosine kinase activity and an SH2 domain. The Abl oncogene is implicated in several human leukemias including 90-95% of chronic myelocytic leukemia (CML), 20-25% of adult acute lymphoblastic leukemia (ALL) and 2-5% of pediatric ALL. In these leukemias the c-Abl proto-oncogene undergoes a (9;22) chromosomal translocation producing the Philadelphia (Ph1) chromosome. The molecular consequence of this translocation is the generation of a chimeric Bcr/c-Abl mRNA encoding activated Abl protein-tyrosine kinase. The Bcr gene has been shown to encode a GTPase-activating protein (GAP) specific for the Ras-related GTP-binding protein, p21rac.
• Synonyms: c-ABL, p150, JTK7, bcr/abl

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors
• Protein Pathways: Axon guidance, Cell cycle, Chronic myeloid leukemia, ErbB signaling pathway, Neurotrophin signaling pathway, Pathogenic Escherichia coli infection, Pathways in cancer, Viral myocarditis