Aromatase (CYP19A1) Rabbit Polyclonal Antibody

CAT#: AP20542PU-N

Aromatase (CYP19A1) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of CYP19A1 Antibody (Cat.-No.: AP20542PU-N) in extracts from 293 cells.

• 

  Immunohistochemistry analysis of CYP19A1 Antibody (Cat.-No.: AP20542PU-N) in Paraffin-Embedded Human brain tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000
  Immunofluorescence: 1/50-1/200
  Immunohistochemistry on Paraffin Sections: 1/50-1/200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of CYP19A1 protein.
  (region surrounding Lys243)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Predicted Protein Size: ~ 58 kDa
• Gene Name: Homo sapiens cytochrome P450 family 19 subfamily A member 1 (CYP19A1), transcript variant 1
• Database Link:
  Entrez Gene 13075 MouseEntrez Gene 25147 RatEntrez Gene 1588 Human
• Background: P450 enzymes constitute a family of monooxygenase enzymes that are involved in the metabolism of a wide array of endogenous and xenobiotic compounds. P450 enzymes can be classified, based on their sequence similarities, into distinct subfamilies, which include CYP1A and CYP2A. Other P450 family members include CYP19, also designated aromatase (P450arom), which catalyzes the conversion of C19 steroids to estrogens in various tissues, including placent,a gonads, adipose tissue, skin and brain. CYP19 expression is controlled by hormonally regulated promoters in different tissues and increased aromatase activity is associated with familial gynecomastia. Also, a polymorphic allele of CYP19 (repeat (TTTA)12) is present in a majority of breast cancer patients. P450 cholesterol 7α-hydroxylase, CYP7A1, is the rate limiting enzyme of bile acid synthesis in the liver, and its expression is mediated by the bile acid receptor FXR. CYP27A1 catalyzes vitamin D 25-hydroxylation and is localized to the mitochondria in kidney and liver.
• Synonyms: Cytochrome P450 19A1, CYPXIX, Estrogen synthetase, P450AROM, CYP19A1, ARO1, CYAR, CYP19

Reference Data

• Protein Families: Druggable Genome, P450
• Protein Pathways: Androgen and estrogen metabolism, Metabolic pathways