Granzyme H (GZMH) Rabbit Polyclonal Antibody

CAT#: AP20560PU-N

Granzyme H (GZMH) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of Granzyme H antibody in extracts from K562 cells.

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  Western blot (WB) analysis of Granzyme H antibody at 1/500 dilution. Lane 1: MCF-7 whole cell lysate. Lane 2: Raw264.7 whole cell lysate. Lane 3: H9C2 whole cell lysate.

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  Immunohistochemistry (IHC) analyzes of Granzyme H antibody in paraffin-embedded human pancreas tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
  Immunofluorescence: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 50-100 of Human Granzyme H.
• Specificity: This antibody detects endogenous levels of Granzyme H protein.
  (region surrounding Gly75)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 21, 27 kDa
• Gene Name: Homo sapiens granzyme H (cathepsin G-like 2, protein h-CCPX) (GZMH), transcript variant 1
• Database Link:
  Entrez Gene 2999 Human
• Background: The granzyme family of proteins belong to the larger peptidase S1 family. Granzyme A and granzyme B are serine proteases that facilitate apoptotic signaling in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Within the granules of activated CTLs, granzyme A and granzyme B are processed and converted to their active forms by the lysosomal cysteine protease cathepsin C. Once cleaved, these active proteases target distinct substrates for proteolysis and, thereby, mediate apoptosis through two different pathways. Granzyme H, also designated cytotoxic T-lymphocyte proteinase, cathepsin G-like 2 (CTSGL2) or cytotoxic serine protease C (CSP-C), contains one peptidase S1 domain. Granzyme H localizes to cytoplasmic granules of cytolytic T-lymphocytes and is important for target cell lysis in cell-mediated immune responses
• Synonyms: CGL2, CTSGL2, CCP-X, Cytotoxic serine protease C, CSP-C

Reference Data

• Protein Families: Druggable Genome, Protease