TRAF3 Rabbit Polyclonal Antibody

CAT#: AP20591PU-N

TRAF3 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of TRAF3 Antibody Cat.-No AP20591PU-N at 1/500 dilution. Lane 1: MCF-7 whole cell lysate. Lane 2: HEK293T whole cell lysate. Lane 3: NIH-3T3 whole cell lysate. Lane 4: Mouse lung tissue lysate. Lane 5: Rat colon tissue lysate.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of TRAF3 protein.
  (region surrounding Ser289)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
  Preservative: 15 mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 68 kDa
• Gene Name: Homo sapiens TNF receptor associated factor 3 (TRAF3), transcript variant 3
• Database Link:
  Entrez Gene 22031 MouseEntrez Gene 362788 RatEntrez Gene 7187 Human
  UniProt ID Q13114
• Background: Tumor necrosis factor (TNF)-activated cell signaling is mediated primarily through the TNF receptor 1 (TNF-R1) and, to a lesser extent, TNF-R2. Both TNF receptors are members of the expanding TNF receptor superfamily which includes the Fas antigen and CD40. Potential insight into an understanding of TNF receptor-mediated signaling was provided by the identification of two related proteins, TRAF1 and TRAF2 (for TNF receptor-associated factors 1 and 2, respectively). Both function to form heterodimeric complexes and associate with the cytoplasmic domain of TNF-R2. A third member of this protein family, alternatively designated CD40 bp, CRAF1, LAP1 or TRAF3, has been identified and shown to associate with the cytoplasmic domain of CD40. The similarity between a specific region of TRAF3 with regions of TRAF1 and TRAF2 define a “TRAF-C” domain that is necessary and sufficient for CD40 binding and homodimerization.
• Synonyms: TNF receptor-associated factor 3, CAP1, CAP-1, LMP1-associated protein, LAP1, CD40-binding protein, CD40BP

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Pathways in cancer, RIG-I-like receptor signaling pathway, Small cell lung cancer, Toll-like receptor signaling pathway