GJC1 Rabbit Polyclonal Antibody

CAT#: AP20594PU-N

GJC1 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of Connecin 45 antibody (Cat.-No.: AP20594PU-N) at 1/500 dilutionLane 1: Hela whole cell lysateLane 2: sp2/20 whole cell lysateLane 3: H9C2 whole cell lysate

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  Immunohistochemistry analysis of Connexin-45 antibody (Cat.-No.: AP20594PU-N) in paraffin-embedded Human brain tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/50-1/200.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of Connexin-45 protein.
  (region surrounding asn361)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Predicted Protein Size: ~45 kDa
• Gene Name: Homo sapiens gap junction protein gamma 1 (GJC1), transcript variant 2
• Database Link:
  Entrez Gene 14615 MouseEntrez Gene 266706 RatEntrez Gene 10052 Human
• Background: Connexin proteins share a common topology of four transmembrane α-helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20 connexin isoforms produces channels with distinct permeabilities and electrical and chemical sensitivities; therefore, one connexin usually cannot fully substitute for another. Consequently, a wide variety of malignant phenotypes associate with decreased connexin expression and gap junction communication, dependent on the particular connexin that is effected. For instance, downregulation of connexin 45 in the left ventricle associates with end-stage heart failure due both to ischaemic cardiomyopathy and idiopathic dilated cardiomyopathy.
• Synonyms: Gap junction gamma-1 protein, Gap junction alpha-7 protein, Connexin-45, GJA7

Reference Data

• Protein Families: Ion Channels: Other, Transmembrane