CD63 Rabbit Polyclonal Antibody

CAT#: AP20598PU-N

CD63 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of CD63 antibody (Cat.-No.: AP20598PU-N) at 1/500 dilution Lane 1:HEK293T cell lysate Lane 2:Raw264.7 cell lysate Lane 3:H9C2 cell lysate

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  Immunohistochemistry analysis of CD63 antibody (Cat.-No.: AP20598PU-N) in Paraffin-Embedded Human lung carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/2000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This detects endogenous levels of CD63 protein.
  (region surrounding Lys156)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 17, 23, 26 kDa
• Gene Name: Homo sapiens CD63 molecule (CD63), transcript variant 1
• Database Link:
  Entrez Gene 967 Human
• Background: The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multimolecular complexes with specific integrins. The tetraspanin CD63 (also known as LAMP-3, Melanoma-associated antigen ME491, TSPAN30, MLA1 and OMA81H) is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome.
• Synonyms: OMA81H, Granulophysin, Tetraspanin-30, MLA1, TSPAN30, ME491

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Lysosome