ABCG2 Rabbit Polyclonal Antibody

CAT#: AP20605PU-N

ABCG2 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of ABCG2 antibody (Cat.-No.: AP20605PU-N) in extracts from HT-29 cells.

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  Immunohistochemistry (IHC) analyzes of ABCG2 antibody (Cat.-No.: AP20605PU-N) in paraffin-embedded human brain tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of ABCG2 protein.
  (region surrounding asn338)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 72 kDa
• Gene Name: Homo sapiens ATP binding cassette subfamily G member 2 (Junior blood group) (ABCG2), transcript variant 1
• Database Link:
  Entrez Gene 9429 Human
  UniProt ID Q9UNQ0
• Background: ATP-binding cassette (ABC) transporters are an evolutionarily conserved family of proteins that catalyze the transport of molecules across extracellular and intracellular membranes through the energy of ATP hydrolysis. The ABC halftransporter, ABCG2, is also known as placenta-specific ABC transporter and breast cancer resistance protein (BCRP1). ABCG2 confers resistance for a variety of chemotherapeutic agents, including anthracyclines, mitoxantrone, bisantrene and topotecan. Under normal conditions, ABCG2 may serve a protective function by removing toxins from the cell, and plays an important role in regulating stem cell differentiation. ABCG2 is responsible for the side population (SP) phenotype and is widely expressed in a large variety of stem cells, making it an important stem cell marker. ABCG2 may have N-linked glycosylation and may dimerize in vivo. ABCG2 is abundantly expressed in placenta, liver, intestine and stem cells.
• Synonyms: Breast cancer resistance protein 1, ABCP, MXR

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: ABC transporters