SDF1 (CXCL12) Rabbit Polyclonal Antibody

CAT#: AP20632PU-N

SDF1 (CXCL12) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of SDF-1 Antibody in extracts from HepG2 cells.

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  Immunohistochemistry analysis of SDF-1 Antibody in paraffin-embedded human brain tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to amino acids 50-100 of Human SDF-1.
• Specificity: This antibody detects endogenous levels of SDF-1 protein.
  (region surrounding Met93)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~10.0 kDa
• Gene Name: Homo sapiens chemokine (C-X-C motif) ligand 12 (CXCL12), transcript variant 2
• Database Link:
  Entrez Gene 20315 MouseEntrez Gene 24772 RatEntrez Gene 6387 Human
• Background: SDF-1 was originally described as a pre-B cell stimulatory factor, but has now been shown to function as a potent chemoattractant for T cells and monocytes, but not neutrophils. Receptors for the C-X-C family are G protein-coupled, seven-pass, transmembrane domain proteins which include IL-8RA, IL-8RB and fusin (also designated LESTR or CXCR-4). Fusin is highly homologous to the IL-8 receptors, sharing 37% sequence identity at the amino acid level. The IL-8 receptors bind to IL-8, NAP-2 and MSGA, while fusin binds to its cognate ligand, SDF-1. Fusin has been identified as the major co-receptor for T-tropic HIV-1, and SDF-1 has been shown to inhibit HIV-1 infection. Six human SDF-1 isoforms exist due to alternative splicing of CXCL12, the gene encoding SDF-1.
• Synonyms: Stromal cell-derived factor 1, SDF1A, SDF1B, IRH, hIRH, PBSF

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
• Protein Pathways: Axon guidance, Chemokine signaling pathway, Cytokine-cytokine receptor interaction, Leukocyte transendothelial migration