GJB1 Rabbit Polyclonal Antibody

CAT#: AP20645PU-N

GJB1 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of Connexin 32 (L115) Antibody at 1/500 dilution: Lane 1: Hela cell lysate. Lane 2: Raw264.7 cell lysate. Lane 3: PC12 cell lysate.

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  Western blot analysis of Connexin-32 antibody in extracts from LOVO cells.

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  Immunohistochemistry analysis of Connexin-32 antibody in paraffin-embedded human breast carcinoma tissue at 1/100 dilution.

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  Immunohistochemistry analysis of Connexin-32 antibody in paraffin-embedded human lung carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to amino acids 81-130 of Human Connexin 32.
• Specificity: This antibody detects endogenous levels of Connexin-32 protein.
  (region surrounding Leu115)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~32 kDa
• Gene Name: Homo sapiens gap junction protein beta 1 (GJB1), transcript variant 2
• Database Link:
  Entrez Gene 14618 MouseEntrez Gene 29584 RatEntrez Gene 2705 Human
• Background: Gap junctions are aggregations of intercellular channels that directly connect the cytoplasm of adjacent cells. Gap junctions coordinate cellular and organ function in tissues and are involved in metabolic cooperation between cells, synchronization of cellular physiological activities, growth control and developmental regulation. The gap junction channels allow intercellular exchange of ions, nucleotides and small molecules between adjacent cells. Unlike other membrane channels, intercellular channels span two plasma membranes and require the contribution of hemichannels, called connexons, from both participating cells. These channels are permeate to molecules as large as 1 kD, and they have been detected in virtually every cell type in mammals, except mature skeletal muscle, spermatozoa and erythrocytes. Two connexons interact in the extracellular space to form the complete intercellular channel. Each connexon is composed of six similar or identical proteins, which are termed connexins. Connexins (Cx) are a multi-gene family of highly related proteins ranging between molecular weights of 26 and 70 kD. At least a dozen distinct connexin genes have been identified and many are expressed in a tissue-specific manner. Two distinct lineages have been identified in mammals. One termed class I or beta group to which Cx26, Cx30, Cx31, Cx31.1 and Cx32 belong, and the other termed class II or alpha group, represented by Cx33, Cx37, Cx40, Cx43 and Cx46.
• Synonyms: Gap junction beta-1 protein, Connexin-32

Reference Data

• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane