ROCK1 Rabbit Polyclonal Antibody

CAT#: AP20652PU-N

ROCK1 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of Rock-1 antibody (Cat.-No.: AP20652PU-N) in extracts from COS7 cells.

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  Immunohistochemistry analyzes of Rock-1 antibody (Cat.-No.: AP20652PU-N) in paraffin-embedded human brain tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1:50 - 1:200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of Rock-1 protein.
  (region surrounding asn311)
• Formulation: Phosphate buffered saline (PBS), pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen; purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 158 kDa
• Gene Name: Homo sapiens Rho associated coiled-coil containing protein kinase 1 (ROCK1)
• Database Link:
  Entrez Gene 19877 MouseEntrez Gene 81762 RatEntrez Gene 6093 Human
  UniProt ID Q13464
• Background: Rho, the Ras-related small GTPase, is responsible for the regulation of Actinbased cytoskeletal structures including stress fibers, focal adhesions and the contractile RING apparatus. Rho proteins function as molecular switches that are able to turn cytokinesis on and off. Although little is known about signaling downstream of Rho, a host of putative Rho effector proteins have been described, including rhophilin, Rhotekin, citron and the serine/threonine kinase, protein kinase N. Two additional Rho-activated serine/threonine kinases have been described, designated Rock-1 and Rock-2 (also referred to as Roka) for Rho-associated coil-containing protein kinase. Rock-1 and Rock-2 share a structural similarity with myotonic dystrophy kinase.
• Synonyms: ROCK-1, p160ROCK, NY-REN-35

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Axon guidance, Chemokine signaling pathway, Focal adhesion, Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton, TGF-beta signaling pathway, Vascular smooth muscle contraction, Wnt signaling pathway