Apolipoprotein E (APOE) Rabbit Polyclonal Antibody

CAT#: AP20663PU-N

Apolipoprotein E (APOE) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of ApoE Antibody (Cat.-No AP20663PU-N) in extracts from RAW264.7cells.

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  Immunohistochemistry analysis of ApoE Antibody (Cat.-No AP20663PU-N) in paraffin-embedded human lung carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of ApoE protein.
  (region surrounding Met86)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 32 kDa
• Gene Name: Homo sapiens apolipoprotein E (APOE), transcript variant 2
• Database Link:
  Entrez Gene 11816 MouseEntrez Gene 25728 RatEntrez Gene 348 Human
• Background: Apolipoprotein-E (apoE) is a protein component of plasma lipoproteins that mediates the binding, internalization and catabolism of lipoprotein particles. It can serve as a ligand for several lipoprotein receptors, including the LDL (apoB/E) receptor and the hepatic apoE (chylomicron remnant) receptor. apoE is produced in most organs and occurs in all plasma lipoprotein fractions, constituting 10-20% of VLDL (very low density lipoprotein) and 1-2% of HDL (high density lipoprotein). Three major isoforms of apoE have been described in human (E2, E3 and E4) which differ by one to two amino acids. Estrogen receptor has been shown to upregulate apoE gene expression via the ERamediated pathway, indicating a potential role for apoE in atherosclerosis. This is consistent with studies in mice in which plasma apoE levels were raised, thereby protecting the mice from diet-induced atherosclerosis. apoE has also been shown to be a potent inhibitor of proliferation and thus may play a role in angiogenesis, tumor cell growth and metastasis.
• Synonyms: ApoE, Apo-E

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, Secreted Protein, Stem cell - Pluripotency
• Protein Pathways: Alzheimer's disease