NGF Rabbit Polyclonal Antibody

CAT#: AP20675PU-N

NGF rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of NGF Antibody (Cat.-No AP20675PU-N) in extracts from Jurkat cells.

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  Immunohistochemistry analysis of NGF Antibody (Cat.-No AP20675PU-N) in paraffin-embedded human lung carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of NGF protein.
  (region surrounding Leu82)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~27 kDa
• Gene Name: Homo sapiens nerve growth factor (beta polypeptide) (NGF)
• Database Link:
  Entrez Gene 18049 MouseEntrez Gene 310738 RatEntrez Gene 4803 Human
  UniProt ID P01138
• Background: The prototype neurotrophin is nerve growth factor (NGF), originally discovered in the 1950s as a soluble peptide promoting the survival of, and neurite outgrowth from, sympathetic ganglia. Three additional structurally homologous neurotrophic factors have been identified. These include brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and neurotrophin-4 (NT-4) (also designated NT-5). These various neurotrophins stimulate the in vitro survival of distinct, but partially overlapping, populations of neurons. The cell surface receptors through which neurotrophins mediate their activity have been identified. For instance, the Trk A receptor is the preferential receptor for NGF, but also binds NT-3 and NT-4.
• Synonyms: Beta-NGF, NGFB,Beta-nerve growth factor

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Apoptosis, MAPK signaling pathway, Neurotrophin signaling pathway