eNOS (NOS3) (1140-1190) Rabbit Polyclonal Antibody

CAT#: AP20681PU-N

eNOS (NOS3) (1140-1190) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analyzes of NOS3 antibody (Cat.-No.: AP20681PU-N) in extracts from Jurkat cells.

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  Immunohistochemistry (IHC) analyzes of NOS3 antibody (Cat.-No.: AP20681PU-N) in paraffin-embedded human brain tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
  Immunofluorescence: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 1140-1190 of Human NOS3.
• Specificity: This antibody detects endogenous levels of NOS3 protein.
  (region surrounding Ser1170)
• Formulation: Phosphate buffered saline (PBS), pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 140 kDa
• Gene Name: Homo sapiens nitric oxide synthase 3 (NOS3), transcript variant 1
• Database Link:
  Entrez Gene 18127 MouseEntrez Gene 24600 RatEntrez Gene 4846 Human
  UniProt ID P29474
• Background: NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); inducible NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS).eNOS is a calcium/calmodulin dependent enzyme which undergoes several post translational modifications, including acylation with myristate and palmitate, and phosphorylation on numerous residues. As with the other members of the NOS family, eNOS derives the diffusible multifunctional second messenger NO from L arginine through a series of reactions in which L citrulline is an intermediate. eNOS plays an important role in controlling vascular tone, platelet aggregation, and cardiac myocyte function.
• Synonyms: Endothelial NOS, EC-NOS, NOS type III, Constitutive NOS, eNOS

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Arginine and proline metabolism, Calcium signaling pathway, Long-term depression, Metabolic pathways, Pathways in cancer, Small cell lung cancer, VEGF signaling pathway