Ezrin (EZR) Rabbit Polyclonal Antibody

CAT#: AP20686PU-N

Ezrin (EZR) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analyzes of Ezrin antibody (Cat.-No.: AP20686PU-N) in extracts from HELA cells.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of Ezrin protein.
  (region surrounding Gly560)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 15 mM sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 69, 81 kDa
• Gene Name: Homo sapiens ezrin (EZR), transcript variant 2
• Database Link:
  Entrez Gene 22350 MouseEntrez Gene 54319 RatEntrez Gene 7430 Human
  UniProt ID P15311
• Background: Ezrin, Moesin and Radixin belong to a family of highly homologous Actinassociated proteins that are localized just beneath the plasma membrane. The proteins are believed to be involved in the mediation of interactions between cytoskeletal and membrane proteins. Ezrin serves as a major cytoplasmic substrate of various protein-tyrosine kinases, including the epidermal growth factor receptor. Ezrin has also been identified as a cAMP-dependent protein kinase (A-kinase) anchoring protein and designated AKAP78. Moesin and Radixin share over 70% homology with Ezrin and are coexpressed within various cell types. Despite the high degree of homology, the three proteins exhibit a distinct receptorspecific pattern of phosphorylation.
• Synonyms: p81, Cytovillin, Villin-2, EZR, VIL2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton