ZAP70 Rabbit Polyclonal Antibody

CAT#: AP20699PU-N

ZAP70 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analyzes of ZAP-70 antibody (Cat.-No.: AP20699PU-N) in extracts from Jurkat cells.

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  Immunohistochemistry analyzes of ZAP-70 antibody (Cat.-No.: AP20699PU-N) in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
  Immunofluorescence: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of ZAP-70 protein.
  (region surrounding Ser313)
• Formulation: Phosphate buffered saline (PBS), pH 7.2
  State: Aff - Purified
  State: Liquid Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen; purity is > 95% (by SDS-PAGE)
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 70 kDa
• Gene Name: Homo sapiens zeta chain of T cell receptor associated protein kinase 70kDa (ZAP70), transcript variant 1
• Database Link:
  Entrez Gene 22637 MouseEntrez Gene 301348 RatEntrez Gene 7535 Human
• Background: The activation of T lymphocytes by antigens is mediated by the T cell receptor (TCR) which is a multisubunit complex assembled from at least six different genes. The TCR subunits include the Ti α and β chains, the CD3 γ, δ and ε chains and a ζ-containing homodimer or heterodimer. The disulfide-linked Ti α-β heterodimer is responsible for antigen recognition, but the short five amino acid cytoplasmic domains of Ti α and β are unlikely to be sufficient to couple to intracellular signaling pathways. In contrast, the structured features of the CD3 and ζ subunits suggest a role in signal transduction. Of these, the ζ chain, which is expressed as either a homodimer or heterodimer, has a short extracellular domain of only nine amino acids, but a larger 113 amino acid cytoplasmic domain. A tyrosine phosphoprotein, ZAP-70, has been identified that associates with ζ and undergoes tyrosine phosphorylation following TCR stimulation.
• Synonyms: ZAP-70, ZAP 70, SRK

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Natural killer cell mediated cytotoxicity, Primary immunodeficiency, T cell receptor signaling pathway