GJA1 Rabbit Polyclonal Antibody

CAT#: AP20736PU-N

GJA1 rabbit polyclonal antibody, Aff - Purified


USD 407.00

2 Weeks*

Size
    • 100 ug

Product Images

Other products for "GJA1"

Specifications

Product Data
Applications IHC, WB
Recommended Dilution Western blot: 1/500 - 1/1000.
Immunohistochemistry on paraffin sections: 1/50 - 1/200.
Reactivities Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Specificity This antibody detects endogenous levels of Connexin 43 protein.
(region surrounding Arg361)
Formulation Phosphate buffered saline (PBS), pH 7.2
State: Aff - Purified
State: Liquid purified Ig fraction
Preservative: 0.05% sodium azide
Concentration 1.0 mg/ml
Purification Affinity-chromatography using epitope-specific immunogen; purity is > 95% (by SDS-PAGE)
Conjugation Unconjugated
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Predicted Protein Size ~ 43 kDa
Gene Name Homo sapiens gap junction protein alpha 1 (GJA1)
Background The connexins are a group of gap junction proteins which form a hexamer to compose a connexon. Clusters of connexons form a gap junction through which low molecular weight proteins may diffuse from cell to cell. Several mammalian cells with malignant phenotypes exhibit decreased connexin expression and gap junction communication. In Src transformed cells, there is a decrease in gap junctional communication, which appears to be associated with tyrosine phosphorylation of connexin 43. Activated c-Src phosphorylates the C-terminal tail of connexin 43 on Tyr 265, resulting in a stable interaction between both proteins, which leads to inhibition of gap junctional communication. In addition to tyrosine phosphorylation, connexin 43 has also been shown to be phosphorylated on serine in the ab-sence of Src kinases and on both serine and tyrosine in cells expressing Src kinases, such as c-Src and/or pp60v-Src. In human vascular endothelial cells, connexin 43 is posttranslationally modified during mitosis. Mitosis-specific phosphorylation of connexin 43 correlates with the transient loss of gap junction intercellular communication and redistribution of connexin 43.
Synonyms Gap junction alpha-1 protein, Connexin-43, GJAL
Reference Data
Protein Families Druggable Genome, Ion Channels: Other, Transmembrane
Protein Pathways Arrhythmogenic right ventricular cardiomyopathy (ARVC), Gap junction

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.