CREB1 Rabbit Polyclonal Antibody

CAT#: AP20740PU-N

CREB1 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analyzes of CREB antibody (Cat.-No.: AP20740PU-N) in extracts from HeLa cells.

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  Immunohistochemistry (IHC) analyzes of CREB antibody (Cat.-No.: AP20740PU-N) in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of CREB protein.
  (region surrounding Lys123)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 38 kDa
• Gene Name: Homo sapiens cAMP responsive element binding protein 1 (CREB1), transcript variant B
• Database Link:
  Entrez Gene 12912 MouseEntrez Gene 81646 RatEntrez Gene 1385 Human
• Background: Eukaryotic gene transcription is regulated by sequencespecific transcription factors that bind modular cis acting promoter and enhancer elements. The ATF/CREB transcription factor family binds the palindromic cAMP response element (CRE) octanucleotide TGACGTCA. The ATF/CREB family includes CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. This family of proteins contains highly divergent N-terminal domains, but shares a C-terminal leucine zipper for dimerization and DNA binding. Although CREB can bind to DNA in an unphosphorylated state, it cannot activate transcription.Phosphorylation of CREB on Ser 133 by protein kinase A facilitates its interaction with the CREBbinding protein (CBP) and activates the basal transcription complex. CREB functions in neoglucogenesis through interactions with the nuclear coactivator PGC-1. CREB may play a role in the pathogenesis of type II diabetes and dilated cardiomyopathy.
• Synonyms: CREB-1

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Antigen processing and presentation, Huntington's disease, Melanogenesis, Prostate cancer