Caspase 4 (CASP4) Rabbit Polyclonal Antibody

CAT#: AP20779PU-N

Caspase 4 (CASP4) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analyzes of Caspase-4 antibody (Cat.-No.: AP20779PU-N) in extracts from K562 cells.

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  Immunohistochemistry (IHC) analyzes of Caspase-4 antibody (Cat.-No.: AP20779PU-N) in paraffin-embedded human lung carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of Caspase-4 protein.
  (region surrounding Glu124)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~34 kDa
• Gene Name: Homo sapiens caspase 4 (CASP4), transcript variant alpha
• Database Link:
  Entrez Gene 12363 MouseEntrez Gene 114555 RatEntrez Gene 837 Human
• Background: A unique family of cysteine proteases has been described that differs in sequence, structure and substrate specificity from any previously described protease family. This family, termed Ced-3/caspase-1, is comprised of caspase- 1, caspase-2, caspase-3, caspase-4, caspase-6 and caspase-7 (also designated Mch3, ICE-LAP3 or CMH-1), caspase-9, and caspase-10. Ced- 3/caspase-1 family members function as key components of the apoptotic machinery and act to destroy specific target proteins which are critical to cellular longevity. Poly(ADP-ribose) polymerase plays an integral role in surveying for DNA mutations and double strand breaks. Caspase-3, caspase-7 and caspase-9, but not caspase-1, have been shown to cleave the 112 kDa nuclear protein PARP into an 85 kDa apoptotic fragment. Caspase-6, but not caspase-3, has been shown to cleave the nuclear lamins which are critical to maintaining the integrity of the nuclear envelope and cellular morphology. Caspase-10 has been shown to activate caspase-3 and caspase-7 in response to apoptotic stimuli.
• Synonyms: CASP4, ICH2, ICE(rel)-II, Protease ICH-2, Protease TX

Reference Data

• Protein Families: Druggable Genome, Protease