c Kit (KIT) pTyr721 Rabbit Polyclonal Antibody

CAT#: AP20799PU-N

c Kit (KIT) pTyr721 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analyzes of p-c-Kit (pTyr721) antibody (Cat.-No.: AP20799PU-N) in extracts from HepG2 EGF cells.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of p-c-Kit protein, only when phosphorylated at Tyr721.
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 120,145 kDa
• Gene Name: Homo sapiens KIT proto-oncogene receptor tyrosine kinase (KIT), transcript variant 1
• Database Link:
  Entrez Gene 16590 MouseEntrez Gene 64030 RatEntrez Gene 3815 Human
• Background: The c-Kit proto-oncogene has been identified as a member of the receptor tyrosine kinase family and more specifically has been shown to be closely related to the platelet derived growth factor receptor (PDGFR). c-Kit, the normal cellular homolog of the HZ4-feline sarcoma virus transforming gene v-Kit, encodes a transmembrane receptor with a molecular weight of 145- 160 kDa. c-Kit has also been show to be identical with the product of the W locus in mice and as such is integral to the development of mast cells and hematopoiesis. The ligand for the c-Kit receptor (KL) has been identified and shown to be encoded at the murine steel (SI) locus. Two sites on c-Kit are able to bind SH2(CHK), the Tyr 568/570 diphosphorylated sequence and the monophosphorylated Tyr 721 sequence.
• Synonyms: SCFR, KIT

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Protein Kinase, Stem cell - Pluripotency, Transmembrane
• Protein Pathways: Acute myeloid leukemia, Cytokine-cytokine receptor interaction, Endocytosis, Hematopoietic cell lineage, Melanogenesis, Pathways in cancer