FOXO3 pSer253 Rabbit Polyclonal Antibody

CAT#: AP20805PU-N

FOXO3 pSer253 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of Phospho-FoxO3 Antibody (Cat.-No AP20805PU-N) (pSer253) in Raw264.7 whole cell lysate at 1/500 dilution.

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  Immunohistochemistry analysis of Phospho-FoxO3 Antibody (Cat.-No AP20805PU-N) (pSer253) in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of FoxO3 protein when phosphorylated at Serine 253
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 15 mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~97 kDa
• Gene Name: Homo sapiens forkhead box O3 (FOXO3), transcript variant 1
• Database Link:
  Entrez Gene 56484 MouseEntrez Gene 2309 Human
• Background: FOXO3A belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. Forkhead transcription factors of the FoxO subfamily are emerging as a shared component among pathways regulating diverse cellular functions, such as differentiation, metabolism, proliferation, and survival. Their transcriptional output is controlled via a two-tiered mechanism of phosphorylation and acetylation.FOXO3A is a transcriptional activator which triggers apoptosis in the absence of survival factors. It recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. FOXO3a has been shown to induce cell growth arrest to effect tumor suppression and FOXO3A genotype is strongly associated with human longevity.
• Synonyms: FOXO3A, AF6q21 protein

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Chemokine signaling pathway, Endometrial cancer, Neurotrophin signaling pathway, Non-small cell lung cancer