CDC25A pSer75 Rabbit Polyclonal Antibody

CAT#: AP20807PU-N

CDC25A pSer75 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of p-Cdc25A antibody (Cat.-No.: AP20807PU-N) in extracts from Jurkat UV cells.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of Cdc25A protein only when phosphorylated at Ser75.
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Predicted Protein Size: ~ 59 kDa
• Gene Name: Homo sapiens cell division cycle 25A (CDC25A), transcript variant 1
• Database Link:
  Entrez Gene 12530 MouseEntrez Gene 171102 RatEntrez Gene 993 Human
• Background: Cyclin dependent kinases are positively regulated by association with cyclins and negatively regulated by binding to inhibitory subunits. The activity of cyclin dependent kinases is also regulated by the phosphorylation status, which is controlled by the antagonistic action of Wee1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, and CDC25C. These three genes function at different phases of the cell cycle. Whereas CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1 S phase and G2 for CDC25B, CDC25C is predominantly expressed in G2.
• Synonyms: CDC-25A

Reference Data

• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Cell cycle, Progesterone-mediated oocyte maturation