Paxillin (PXN) pTyr31 Rabbit Polyclonal Antibody

CAT#: AP20813PU-N

Paxillin (PXN) pTyr31 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analyzes of p-Paxillin (pTyr31) antibody (Cat.-No.: AP20813PU-N) in extracts from HeLa TNF cells.

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  Immunohistochemistry (IHC) analyzes of p-Paxillin (pTyr31) antibody (Cat.-No.: AP20813PU-N) in paraffin-embedded human tonsil tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of p-Paxillin protein, only when phosphorylated at Tyr31.
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 68 kDa
• Gene Name: Homo sapiens paxillin (PXN), transcript variant 2
• Database Link:
  Entrez Gene 19303 MouseEntrez Gene 360820 RatEntrez Gene 5829 Human
• Background: The effects of some oncogenes, growth factors and neuropeptides are mediated by tyrosine phosphorylation of focal adhesion kinase (FAK) and paxillin cytoskeletal proteins. A rapid increase in tyrosine phosphorylation of paxillin, FAK and Crk-associated substrate (CAS) are prominent early events triggered by many G protein-coupled receptors. In addition to G protein-coupled receptors, Angiotensin IV (Ang IV), protein kinase C and other proteins can also mediate the tyrosine phosphorylation of paxillin. Paxillin must bind FAK for maximal phosphorylation in response to cell adhesion. FAK may function to direct tyrosine phosphorylation of paxillin in the process of transformation by the Src oncogene.
• Synonyms: FLJ16691

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Chemokine signaling pathway, Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, VEGF signaling pathway