SRC pTyr529 Rabbit Polyclonal Antibody

CAT#: AP20819PU-N

SRC pTyr529 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analyzes of p-c-Src antibody (Cat.-No.: AP20819PU-N) in extracts from COS PMA cells.

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  Immunohistochemistry (IHC) analyzes of p-c-Src antibody (Cat.-No.: AP20819PU-N) in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of c-Src protein only when phosphorylated at Tyr529.
• Formulation: Phosphate buffered saline (PBS), pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 60 kDa
• Gene Name: Homo sapiens SRC proto-oncogene, non-receptor tyrosine kinase (SRC), transcript variant 1
• Database Link:
  Entrez Gene 20779 MouseEntrez Gene 83805 RatEntrez Gene 6714 Human
  UniProt ID P12931
• Background: The major translational products of the members of the Src gene family identified to date are membrane-associated tyrosine protein kinases that lack transmembrane and external amino acid sequences, thereby distinguishing this group from the receptor class of tyrosine kinases. Members of this group include c-Src, c-Yes, Fyn, Lck, Lyn, Blk and c-Fgr. The major Src gene encoded protein, pp60Src, is expressed in a broad range of tissue and cell types, although the highest levels of pp60Src are detected in neuronal tissues and platelets. pp60Src may play a role in events associated with both neuronal differentiation and maintenance of mature neuronal cell functions.
• Synonyms: c-Src, pp60c-src

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Stem cell relevant signaling - JAK/STAT signaling pathway
• Protein Pathways: Adherens junction, Endocytosis, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Focal adhesion, Gap junction, GnRH signaling pathway, Tight junction, VEGF signaling pathway