ZAP70 pTyr493 Rabbit Polyclonal Antibody

CAT#: AP20821PU-N

ZAP70 pTyr493 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analyzes of p-ZAP-70 (Cat.-No.: AP20821PU-N) in extracts from Jurkat cells.

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  Immunohistochemistry (IHC) analyzes of p-ZAP-70 antibody (Cat.-No.: AP2082PU-N) in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of ZAP-70 protein only when phosphorylated at Tyr493.
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 70 kDa
• Gene Name: Homo sapiens zeta chain of T cell receptor associated protein kinase 70kDa (ZAP70), transcript variant 1
• Database Link:
  Entrez Gene 22637 MouseEntrez Gene 301348 RatEntrez Gene 7535 Human
• Background: The activation of T lymphocytes by antigens is mediated by the T cell receptor (TCR), which is a multisubunit complex assembled from at least six different genes. The TCR subunits include the Ti alpha and beta chains, the CD3 gamma, delta and epsilon chains and a zeta-containing homodimer or heterodimer. The protein tyrosine kinase ZAP-70 binds to the phosphorylated immunoreceptor tyrosine-base activation motifs (ITAMs) of the TCR zeta chain through two Src homology (SH2) domains. This binding results in the phosphorylation of ZAP-70 on multiple tyrosine residues, including Tyr 292 and Tyr 319. ZAP-70 is autophosphorylated on Tyr 292, which is thought to negatively regulate ZAP-70 function in lymphocytes. Alternatively, ZAP-70 is positively regulated by phosphorylation on Tyr 319, which mediates the SH2-dependent interaction between Lck and ZAP-70.
• Synonyms: ZAP-70, ZAP 70, SRK

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Natural killer cell mediated cytotoxicity, Primary immunodeficiency, T cell receptor signaling pathway