IKB alpha (NFKBIA) pTyr42 Rabbit Polyclonal Antibody

CAT#: AP20824PU-N

IKB alpha (NFKBIA) pTyr42 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of p-IkB-a antibody (Cat.-No.: AP20824PU-N) at 1/500 dilution Lane 1:Jurkat whole cell lysate treated with IL-1Beta Lane 2:H9C2 whole cell lysate treated with LPS Lane 3:RAW264.7 whole cell lysate treated with LPS using p-IkB-a (Y42) pAb (AP20824PU-N)

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  Immunohistochemistry (IHC) analyzes of p-IkappaB-alpha antibody (Cat.-No.: AP20824PU-N) in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of IkappaB-alpha protein only when phosphorylated at Tyr42.
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 36 kDa
• Gene Name: Homo sapiens nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA)
• Database Link:
  Entrez Gene 18035 MouseEntrez Gene 25493 RatEntrez Gene 4792 Human
  UniProt ID P25963
• Background: On the basis of both functional and structural considerations, members of the IkappaB family of proteins can be divided into four groups. The first of these groups, IkappaB-alpha (a 35-37 kDa protein), includes the avian protein pp40 and the mammalian MAD-3, both of which inhibit binding of p50-p65 NFkappaB complex or Rel protein to their cognate binding sites but do not inhibit the binding of p50 homodimer to kappaB sites, suggesting that the IkappaB-alpha family binds to the p65 subunit of p50-p65 heterocomplex through ankyrin repeats. The second member of the IkappaB family is represented by a 45 kDa protein designated IkappaB-beta. The third group of IkappaB proteins is represented by IkappaB-gamma, a 70 kDa protein identical in sequence with the Cterminal domain of the p110 precursor of NFkappaB p50 and expressed predominantly in lymphoid cells.
• Synonyms: I-kappa-B-alpha, MAD3, NFKBI, I kappa B-alpha, IkappaBalpha, IkB-alpha

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Cytosolic DNA-sensing pathway, Epithelial cell signaling in Helicobacter pylori infection, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Pathways in cancer, Prostate cancer, RIG-I-like receptor signaling pathway, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway