EIF4EBP1 pThr46 Rabbit Polyclonal Antibody

CAT#: AP20835PU-N

EIF4EBP1 pThr46 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analyzes of p-4E-BP1 antibody (Cat.-No.: AP20835PU-N) in extracts from MDA-MB-435 EGF cells.

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  Immunohistochemistry (IHC) analyzes of p-4E-BP1 antibody (Cat.-No.: AP20835PU-N) in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of 4E-BP1 protein only when phosphorylated at Thr46.
• Formulation: Phosphate buffered saline (PBS, pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 15 to 20 kDa
• Gene Name: Homo sapiens eukaryotic translation initiation factor 4E binding protein 1 (EIF4EBP1)
• Database Link:
  Entrez Gene 13685 MouseEntrez Gene 116636 RatEntrez Gene 1978 Human
  UniProt ID Q13541
• Background: The multisubunit eukaryotic translation initiation factor (eIF) 4F recruits 40S ribosomal subunits to the 5’ end of mRNA. The eIF4F subunit eIF4E interacts directly with the mRNA 5’ cap structure. Assembly of the eIF4F complex is inhibited by a family of repressor polypeptides, the eIF4E-binding proteins (4E-BPs). 4E-BP1 (also known as PHAS-1) normally binds eIF4E, inhibiting cap-dependent translation. Hyperphosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation. The PI3-kinase/Akt pathway and the FRAP/mTOR kinase regulate 4E-BP1. 4E-BP1 is phosphorylated in vivo on multiple residues and phosphorylation by FRAP/mTOR on Threonine 37 and Threonine 46 of human 4E-BP1 may prime it for subsequent phosphorylation at sites including Serine 65 and Threonine 70. The corresponding rat residues include Threonine 36, Threonine 45, Serine 64 and Threonine 69.
• Synonyms: PHAS-I, PHAS-1, PHAS1

Reference Data

• Protein Pathways: Acute myeloid leukemia, ErbB signaling pathway, Insulin signaling pathway, mTOR signaling pathway