EIF4E pSer209 Rabbit Polyclonal Antibody

CAT#: AP20836PU-N

EIF4E pSer209 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of p-eIF4E antibody (Cat.-No.: AP20836PU-N) in extracts from NIH/3T3 FBS cells.

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  Immunohistochemistry (IHC) analyzes of p-eIF4E (Cat.-No.: AP20836PU-N) in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of eIF4E protein only when phosphorylated at Ser209.
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 25 kDa
• Gene Name: Homo sapiens eukaryotic translation initiation factor 4E (EIF4E), transcript variant 1
• Database Link:
  Entrez Gene 13684 MouseEntrez Gene 117045 RatEntrez Gene 1977 Human
  UniProt ID P06730
• Background: The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF4F exists in vitro as a trimeric complex of eIF4G, eIF4E, and eIF4A. Together, the complex allows ribosome binding to mRNA by inducing the unwinding of mRNA secondary structures. eIF4E is a 25 kDa protein that binds to the mRNA “cap” during an early step in the initiation of protein synthesis. eIF4A is a 46 kDa protein that acts as an ATP-dependent RNA helicase. eIF4G is 154 kDa protein that acts as a bridge between eIF4E, eIF4A, and the eIF3 complex.
• Synonyms: eIF-4F 25 kDa subunit, EIF-4E, EIF4EL1, EIF4F

Reference Data

• Protein Pathways: Insulin signaling pathway, mTOR signaling pathway