Stathmin 1 (STMN1) pSer25 Rabbit Polyclonal Antibody

CAT#: AP20838PU-N

Stathmin 1 (STMN1) pSer25 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analyzes of p-Op18 antibody (Cat.-No.: AP20838PU-N) in extracts from Jurkat cells.

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  Immunohistochemistry (IHC) analyzes of p-Op18 antibody (Cat.-No.: AP20838PU-N) in paraffin-embedded human thymus gland tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western Blot: 1/500 - 1/1000.
  Immunohistochemistry: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of Op18 protein only when phosphorylated at Ser25.
• Formulation: Phosphate buffered saline (PBS, pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 19 kDa
• Gene Name: Homo sapiens stathmin 1 (STMN1), transcript variant 3
• Database Link:
  Entrez Gene 16765 MouseEntrez Gene 29332 RatEntrez Gene 3925 Human
• Background: Oncoprotein 18 (Op18) is a regulator of microtubule (MT) dynamics. Op18 is a target for both cell cycle and cell surface receptor-coupled kinase systems, and phosphorylation of Op18 on specific combinations of amino acid residues has been shown to switch off MT-destabilizing activity of Op18. Induced expression of the catalytic subunit of cAMPdependent protein kinase (PKA) results in a dramatic increase in cellular MT polymer content concomitant with phosphorylation and partial degradation of Op18. Phosphorylation of Op18 on two specific sites, Serine 16 and Serine 63, is necessary and sufficient for PKA to switch off Op18 activity in intact cells. PKA phosphorylation down-regulates the MT-destabilizing activity of Op18, which in turn promotes increased Tubulin polymerization. Op18 has the potential to regulate the MT system in response to external signals such as cAMP-linked agonists.
• Synonyms: LAP18, OP18, Phosphoprotein p19, pp19, Leukemia-associated phosphoprotein p18, Oncoprotein 18, Op18, pp17, Prosolin, Metablastin, Protein Pr22

Reference Data

• Protein Pathways: MAPK signaling pathway