JNK1 (MAPK8) pThr183 Rabbit Polyclonal Antibody

CAT#: AP20849PU-N

JNK1 (MAPK8) pThr183 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of p-JNK1/2/3 antibody (Cat.-No.: AP20849PU-N) at 1/500 dilution Lane 1:MCF-7 whole cell lysate treated with PMA Lane 2:NIH-3T3 whole cell lysate treated with UV Lane 3:PC12 whole cell lysate treated with PMA

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  Immunohistochemistry (IHC) analyzes of p-JNK1/2/3 antibody (Cat.-No.: AP20849PU-N) in paraffin-embedded human colon carcinoma tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
  Immunofluorescence: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of p-JNK1/2/3 protein only when phosphorylated at Thr183.
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 15 mM sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 46,54 kDa
• Gene Name: Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant JNK1-a1
• Database Link:
  Entrez Gene 26419 MouseEntrez Gene 116554 RatEntrez Gene 5599 Human
  UniProt ID P45983
• Background: JNKs (c-Jun N-terminal kinases) belong to a family of MAP kinases that are involved in a variety of cellular processes, including transcriptional regulation and cellular proliferation, differentiation and development. JNK2 (c-Jun Nterminal kinase 2) and JNK3 (c-Jun N-terminal kinase 3) are 424 and 464 amino acid proteins, respectively, that each contain one protein kinase domain and use magnesium as a cofactor to catalyze the phosphorylation of target proteins, thereby playing a role in a variety of events throughout the cell. Both JNK2 and JNK3 exist as multiple alternatively spliced isoforms and are subject to post-translational phosphorylation on Thr 183 and Thr 221, respectively, an event which activates JNK2/JNK3 enzymatic activity. Defects in the gene encoding JNK3 are a cause of epileptic encephalopathy of the Lennox-Gastaut type, a group of epileptic disorders characterized by severe psychomotor delay and seizures.
• Synonyms: MAPK8, Mitogen-activated protein kinase 8, c-Jun N-terminal kinase 1, JNK-46, JNK-1, PRKM8, JNK2, JNK3

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase
• Protein Pathways: Adipocytokine signaling pathway, Colorectal cancer, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Fc epsilon RI signaling pathway, Focal adhesion, GnRH signaling pathway, Insulin signaling pathway, MAPK signaling pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway, Type II diabetes mellitus, Wnt signaling pathway