S6K1 (RPS6KB1) pSer434 Rabbit Polyclonal Antibody

CAT#: AP20852PU-N

S6K1 (RPS6KB1) pSer434 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analyzes of p-p70 S6 kinase alpha antibody (Cat.-No.: AP20852PU-N) in extracts from k562 insulin cells.

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  Immunohistochemistry (IHC) analyzes of p-p70 S6 kinase alpha antibody (Cat.-No.: AP20852PU-N) in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of p-p70 S6 kinase alpha protein only when phosphorylated at Ser411.
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 50% glycerol
  Preservative: 0.09% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~59,70,85 kDa
• Gene Name: Homo sapiens ribosomal protein S6 kinase B1 (RPS6KB1), transcript variant 1
• Database Link:
  Entrez Gene 72508 MouseEntrez Gene 83840 RatEntrez Gene 6198 Human
• Background: In studies to elucidate key regulatory pathways in signal transduction, several protein serine/threonine (Ser/Thr) kinases have been identified. Included among such kinases are two distinct families of 40S Ribosomal Protein S6 Ser/Thr kinases present in somatic animal cells, designated p70 S6 kinase and p90 Rsk kinase. p90 Rsk kinase is maximally activated within minutes of addition of growth factors or phorbol ester to cultured cells followed by activation of p70 S6 kinase. Both enzymes are regulated by serine/threonine phosphorylation, suggesting that specific kinases may exist upstream in the signaling pathway that regulate these kinases. In fact, evidence suggests that one such family of activating enzymes includes the members of the ERK MAP kinase family. The ERK MAP kinases are, in turn, regulated by phosphorylation at threonine and tyrosine residues by a protein kinase designated MEK.
• Synonyms: Ribosomal protein S6 kinase I, S6K1, p70 S6 kinase alpha, p70 S6K-alpha, p70 S6KA, Serine/threonine-protein kinase 14A

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Acute myeloid leukemia, ErbB signaling pathway, Fc gamma R-mediated phagocytosis, Insulin signaling pathway, mTOR signaling pathway, TGF-beta signaling pathway