Cortactin (CTTN) pTyr421 Rabbit Polyclonal Antibody

CAT#: AP20857PU-N

Cortactin (CTTN) pTyr421 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of Cortactin antibody (Cat.-No AP20857PU-N) in extracts from 3T3 H2O2 cells

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  Immunohistochemistry analysis of Cortactin antibody (Cat.-No AP20857PU-N) in paraffin-embedded human prostate carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of Cortactin protein only when phosphorylated at Tyr421.
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 85.0 kDa
• Gene Name: Homo sapiens cortactin (CTTN), transcript variant 1
• Database Link:
  Entrez Gene 13043 MouseEntrez Gene 60465 RatEntrez Gene 2017 Human
• Background: Cortactin (also designated Ems-1) is a filamentous Actin (FActin) binding protein. It has also been shown to be a substrate for Src p60. Cortactin contains tandem 37 amino acid repeats at the amino-terminus and an SH3 domain at the carboxy-terminus. The tandem repeats appear to be necessary for F-Actin binding. Tyrosine phosphorylation of Cortactin by Src p60 results in diminished F-Actin binding to Cortactin and reduced F-Actin cross-linking activity. Cortactin has also been shown to be phosphorylated in response to FGF-1. Cortactin exhibits abundant expression in megakaryocytes and platelets, and it may play a role in the maturation of megakaryocytes.
• Synonyms: CTTN, EMS1, Amplaxin, Oncogene EMS1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Pathogenic Escherichia coli infection, Tight junction