PPP1R14A pThr38 Rabbit Polyclonal Antibody

CAT#: AP20858PU-N

PPP1R14A pThr38 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of p-CPI-17 antibody (Cat.-No.: AP20858PU-N) in extracts from RAW264.7 cell

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  Immunohistochemistry (IHC) analyzes of p-CPI-17 antibody (Cat.-No.: AP20858PU-N) in paraffin-embedded human heart tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of CPI-17 protein only when phosphorylated at Thr38.
• Formulation: Phosphate buffered saline (PBS), pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 22 kDa
• Gene Name: Homo sapiens protein phosphatase 1 regulatory inhibitor subunit 14A (PPP1R14A), transcript variant 1
• Database Link:
  Entrez Gene 68458 MouseEntrez Gene 114004 RatEntrez Gene 94274 Human
  UniProt ID Q96A00
• Background: CPI-17 is a phosphorylation-dependent inhibitory protein for smooth muscle Myosin phosphate. CPI-17 was originally identified as a 17 kDa PKC-potentiated inhibitory protein of protein phosphatase-1, which is dominantly expressed in smooth muscle. Phosphorylation at Threonin 38, in vitro, by PKC or Rho-kinase enhances the inhibitory potency toward myosin phosphatase. CPI-17 is also phosphorylated at Threonine 38 by protein kinase N and might be involved in the calcium sensitization of smooth muscle cont-raction as a downstream effector of Rho and/or arachidonic acid. CPI-17 is dually phosphorylated at Serine 12 and Threonine 38 by a MYPT-associated kinase, M110 kinase.
• Synonyms: Protein phosphatase 1 regulatory subunit 14A, CPI-17, CPI17, PPP1INL

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Vascular smooth muscle contraction